Journal: Nutrition Research and Practice
Article Title: Dopamine and serotonin alterations by Hizikia fusiformis extracts under in vitro cortical primary neuronal cell cultures
doi: 10.4162/nrp.2023.17.3.408
Figure Lengend Snippet: HF, Hizikia fusiformis extract treatment; VEH, vehicle treatment (control); DRD1-5, dopamine D1–5 receptors; DAT, dopamine transporter; DAPI, 4′,6-diamidino-2-phenylindole; NF-L, neurofilament light chain; SERT, serotonin transporter; TH, tyrosine hydroxylase; TPH, tryptophan hydroxylase; SYP, synaptophysin; 5HT1A and 1B, serotonin 1A and 1B receptors; MAP2, microtubule-associated protein 2.
Article Snippet: In this study, we used primary antibodies for DA transmission: mouse monoclonal anti-tyrosine hydroxylase (TH; Abcam; catalog #ab129991), rabbit polyclonal anti-DAT (Santa Cruz; catalog #sc-14002), rabbit polyclonal anti-dopamine receptor D1 (DRD1; Santa Cruz; catalog #sc-14001), mouse monoclonal anti-DRD2 (Santa Cruz; catalog #sc-5303), mouse monoclonal anti-DRD3 (Santa Cruz; catalog #sc-136170), rabbit polyclonal anti-DRD4 (Elabscience; catalog #E-AB-31153), and rabbit polyclonal anti-DRD5 (Mybiosource; catalog #MBS2516950) antibodies; those for 5-HT transmission: rabbit polyclonal anti-5HT1A (Elabscience; catalog #E-AB-32950), rabbit polyclonal anti-5HT1B (Abcam; catalog #ab13896), anti-tryptophan hydroxylase (TPH; catalog #E-AB-16937), and mouse monoclonal anti-SERT (Santa Cruz; catalog #sc-33724) antibodies; and those for neuronal structures: mouse monoclonal anti-synaptophysin (SYP; Sigma-Aldrich; catalog #S5768), mouse monoclonal anti-microtubule-associated protein 2 (MAP2; Santa Cruz; catalog #sc-32791), and mouse monoclonal neurofilament-light chain (NF-L; Santa Cruz; catalog #sc-20012) antibodies.
Techniques: